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Rapid Optical Clearing for Semi-High-Throughput Analysis of Tumor Spheroids
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
Rapid Optical Clearing for Semi-High-Throughput Analysis of Tumor Spheroids

Rapid Optical Clearing for Semi-High-Throughput Analysis of Tumor Spheroids

English

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2,438 Views

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08:12 min

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August 23, 2022

DOI:

10.3791/64103-v

DOI:
10.3791/64103-v

•

08:12 min
August 23, 2022

•

10 Views
, ,
1The University of Queensland Diamantina Institute,The University of Queensland, 2School of Mathematical Sciences,Queensland University of Technology

Summary

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Tumor spheroids are becoming increasingly utilized to assess tumor cell-microenvironment interactions and therapy response. The present protocol describes a robust but simple method for semi-high-throughput imaging of 3D tumor spheroids using rapid optical clearing.

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Cite this Article

Gunasingh, G., Browning, A. P., Haass, N. K. Rapid Optical Clearing for Semi-High-Throughput Analysis of Tumor Spheroids. J. Vis. Exp. (186), e64103, doi:10.3791/64103 (2022).

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