Tumor spheroids are becoming increasingly utilized to assess tumor cell-microenvironment interactions and therapy response. The present protocol describes a robust but simple method for semi-high-throughput imaging of 3D tumor spheroids using rapid optical clearing.
Gunasingh, G., Browning, A. P., Haass, N. K. Rapid Optical Clearing for Semi-High-Throughput Analysis of Tumor Spheroids. J. Vis. Exp. (186), e64103, doi:10.3791/64103 (2022).