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Imagerie unicellulaire du cerveau entier et analyse de cerveaux de souris néonatales intactes à l’aide de l’IRM, du nettoyage des tissus et de la microscopie à feuille de lumière
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1UNC Neuroscience Center,University of North Carolina, Chapel Hill, 2Department of Genetics,University of North Carolina, Chapel Hill, 3Department of Psychiatry,University of North Carolina, Chapel Hill, 4Center for Animal Magnetic Resonance Imaging,The University of North Carolina at Chapel Hill, 5Biomedical Research Imaging Center,The University of North Carolina at Chapel Hill, 6Department of Neurology,The University of North Carolina at Chapel Hill, 7Department of Computer Science,The University of North Carolina at Greensboro
Chapters
- 00:05Introduction
- 00:48Light-Sheet Imaging
- 02:25Image Processing Using NuMorph
- 04:12Image Analysis
- 06:26Results: Quantification of Nuclear Markers Within Mouse Brain After Imaging on a Light-Sheet Microscope Using NuMorph
- 07:57Conclusion
Ce protocole décrit les méthodes d’imagerie par résonance magnétique, d’effacement et d’immunomarquage de cerveaux de souris intacts à l’aide d’iDISCO+, suivies d’une description détaillée de l’imagerie à l’aide de la microscopie à feuille de lumière et des analyses en aval à l’aide de NuMorph.
Tags
Whole-brainSingle-cellImagingAnalysisIntactNeonatalMouseBrainMRILight-sheet MicroscopyImage AnalysisNuclei QuantificationComputational PipelinePreprocessingCell DetectionImaging TimeComputational ResourcesSample MountingSample OrientationZoom MagnificationLight SheetNumerical ApertureDynamic FocusingLaser PowerLight Sheet WidthTile OverlapCondaNuMorphMATLABImage ProcessingIntensity Adjustment
