The present protocol describes a two-point injection of lysophosphatidylcholine via a stereotaxic frame to generate a stable and reproducible demyelination model in mice.
Pang, X., Chen, M., Chu, Y., Tang, Y., Qin, C., Tian, D. A Stably Established Two-Point Injection of Lysophosphatidylcholine-Induced Focal Demyelination Model in Mice. J. Vis. Exp. (183), e64059, doi:10.3791/64059 (2022).