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A Stably Established Two-Point Injection of Lysophosphatidylcholine-Induced Focal Demyelination Model in Mice
JoVE 杂志
神经科学
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JoVE 杂志 神经科学
A Stably Established Two-Point Injection of Lysophosphatidylcholine-Induced Focal Demyelination Model in Mice

A Stably Established Two-Point Injection of Lysophosphatidylcholine-Induced Focal Demyelination Model in Mice

DOI:
10.3791/64059-v

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04:55 min

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May 11, 2022

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, , , , ,
1Department of Neurology, Tongji Hospital, Tongji Medical College,Huazhong University of Science and Technology

Chapters

  • 00:05Introduction
  • 00:43Surgical Preparation and Surgical Procedure
  • 02:34Results: The Effects of Two-Point Injection of the LPC in Demyelination, Myelin Regeneration, and the Spatial Memory of Mice
  • 04:30Conclusion

Summary

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The present protocol describes a two-point injection of lysophosphatidylcholine via a stereotaxic frame to generate a stable and reproducible demyelination model in mice.

Tags

Focal DemyelinationLysophosphatidylcholineCorpus CallosumStereotaxic InjectionMultiple SclerosisMouse ModelLuxol Fast BlueDMBPWhite Matter Lesions

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