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Journal
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Immunology and Infection
/
厌氧菌小鼠阴道定植模型
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
Models of Murine Vaginal Colonization by Anaerobically Grown Bacteria
Please note that all translations are automatically generated.
Click here for the English version.
厌氧菌小鼠阴道定植模型
DOI:
10.3791/64032-v
•
08:53 min
•
May 25, 2022
•
Sydney R. Morrill
2,3
,
Kavita Agarwal
3
,
Sudeshna Saha
3
,
Warren G. Lewis
3
,
Nicole M. Gilbert
,
Amanda L. Lewis
3
1
Division of Biology and Biomedical Sciences
,
Washington University School of Medicine
,
2
Department of Obstetrics, Gynecology, and Reproductive Sciences
,
University of California
,
3
Glycobiology Research and Training Center
,
UCSD
,
4
Department of Pediatrics
,
Washington University School of Medicine
Chapters
00:05
Introduction
00:52
Preparation of Inoculum
02:08
Vaginal Pre-Lavage and Inoculation with
Gardnerella
03:54
Dissection and Tissue Homogenization
07:26
Results: Enumeration of Bacteria Colonizing Mouse Vagina
08:23
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议提出了用厌氧培养的人阴道细菌阴道定植的小鼠模型。我们专注于
阴道加德纳
菌,同时包括
对双普雷沃氏
菌和核
梭杆菌
的建议。该方案也可以用作阴道接种和其他厌氧生长细菌的可行回收的指南。
Tags
Anaerobic Bacteria
Vaginal Colonization
Murine Model
Inoculation
Vaginal Lavage
Colony-forming Units
Obligate Anaerobes
Fastidious Bacteria
Spectrophotometry
Anaerobic Conditions
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