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Assessing Hepatic Metabolic Changes During Progressive Colonization of Germ-free Mouse by 1H NMR Spectroscopy
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Chapters
- 00:05Title
- 01:12Colonization of Germ-free Animals and Sample Collection
- 02:071H NMR Acquisition of Urine Microvolume
- 03:25Collection of Liver Biopsy
- 04:091H High Resolution Magic Angle Spinning (HR MAS) NMR of Liver Tissue Biopsy: Sample Preparation
- 05:55Urinary and Hepatic Metabolic Profiling through 1H NMR and 1H HR MAS NMR Spectra
- 07:32Conclusion
A progressive colonization procedure is described to further assess its impact on the host hepatic metabolism. Colonization is monitored non invasively by evaluating the urinary excretion of microbial co-metabolites by NMR-based metabolic profiling while hepatic metabolism is assessed by High Resolution Magic Angle Spinning (HR MAS) NMR profiling of intact biopsy.
