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A Guide to Examining Intramuscular Fat Formation and its Cellular Origin in Skeletal Muscle
JoVE Journal
Developmental Biology
This content is Free Access.
JoVE Journal Developmental Biology
A Guide to Examining Intramuscular Fat Formation and its Cellular Origin in Skeletal Muscle

A Guide to Examining Intramuscular Fat Formation and its Cellular Origin in Skeletal Muscle

DOI:
10.3791/63996-v

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09:19 min

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May 26, 2022

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, ,
1Department of Pharmacology and Therapeutics,University of Florida College of Medicine, 2Myology Institute,University of Florida College of Medicine

Chapters

  • 00:05Introduction
  • 00:51Injury of Tibialis Anterior (TA) Muscle
  • 01:28Tissue Harvest
  • 03:43Imaging of Intramuscular Fat
  • 04:26Adipogenic Gene Expression Analysis Using RT-qPCR
  • 07:11Results: Visualization and Quantification of Intramuscular Fat
  • 08:51Conclusion

Summary

Automatic Translation

Automatic Translation

The replacement of healthy muscle tissue with intramuscular fat is a prominent feature of human diseases and conditions. This protocol outlines how to visualize, image, and quantify intramuscular fat, allowing the rigorous study of the mechanisms underlying intramuscular fat formation.

Tags

Intramuscular FatSarcopeniaNeuromuscular DiseasesAdipocyte MorphologySkeletal MuscleDuchenne Muscular DystrophyGlycerolTibialis AnteriorHistology

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