The replacement of healthy muscle tissue with intramuscular fat is a prominent feature of human diseases and conditions. This protocol outlines how to visualize, image, and quantify intramuscular fat, allowing the rigorous study of the mechanisms underlying intramuscular fat formation.
Johnson, C. D., Zhou, L. Y., Kopinke, D. A Guide to Examining Intramuscular Fat Formation and its Cellular Origin in Skeletal Muscle. J. Vis. Exp. (183), e63996, doi:10.3791/63996 (2022).