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从未受干扰和受伤的小鼠骨骼肌中分离和培养纤维脂肪祖细胞和肌肉干细胞的FACS-分离和培养
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JoVE Journal
Developmental Biology
FACS-Isolation and Culture of Fibro-Adipogenic Progenitors and Muscle Stem Cells from Unperturbed and Injured Mouse Skeletal Muscle
Please note that all translations are automatically generated.
Click here for the English version.
从未受干扰和受伤的小鼠骨骼肌中分离和培养纤维脂肪祖细胞和肌肉干细胞的FACS-分离和培养
DOI:
10.3791/63983-v
•
11:25 min
•
June 08, 2022
•
Giulia Riparini
,
James M. Simone
,
Vittorio Sartorelli
1
Laboratory of Muscle Stem Cells and Gene Regulation, National Institute of Arthritis, Musculoskeletal, and Skin Diseases (NIAMS)
,
National Institutes of Health (NIH)
,
2
Flow Cytometry Section, National Institute of Arthritis, Musculoskeletal, and Skin Diseases (NIAMS)
,
National Institutes of Health (NIH)
Chapters
00:04
Introduction
01:04
Hind Limb Muscle Harvesting
03:11
Mechanical and Enzymatic Muscle Digestion
05:04
Generation of Mononucleated Cells
07:03
Antibody Staining for Flow Cytometry
08:49
Results: FACS-Assisted Simultaneous Isolation and Culture of FAPs and MuSCs from Mouse Limb Muscles
10:37
Conclusion
Summary
Automatic Translation
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Automatic Translation
纤维脂肪祖细胞(FAPs)和肌肉干细胞(MuSCs)的精确鉴定对于研究它们在生理和病理条件下的生物学功能至关重要。该协议为从成年小鼠肌肉中分离,纯化和培养FAP和MuSC提供了指南。
Tags
FACS
Fibro-adipogenic Progenitors
Muscle Stem Cells
Skeletal Muscle
Isolation
Culture
Fluorescence-activated Cell Sorting
Muscle Injury
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