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Reconstitución de corticidades mínimas de actina atadas a la membrana en bicapas lipídicas soportadas
JoVE Journal
Biology
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JoVE Journal Biology
Reconstitution of Membrane-Tethered Minimal Actin Cortices on Supported Lipid Bilayers
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Reconstitución de corticidades mínimas de actina atadas a la membrana en bicapas lipídicas soportadas

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1,814 Views

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11:55 min

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July 12, 2022

DOI:

10.3791/63968-v

DOI:
10.3791/63968-v

•

11:55 min
July 12, 2022

•

5 Views
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1Centre for Mechanochemical Cell Biology and Division of Biomedical Sciences, Warwick Medical School,University of Warwick, 2National Centre for Biological Sciences,Tata Institute of Fundamental Research

Summary

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Este protocolo describe la formación de bicapas lipídicas soportadas y la adición de filamentos citoesqueléticos y proteínas motoras para estudiar la dinámica de las redes citoesqueléticas reconstituidas y atadas a la membrana utilizando microscopía de fluorescencia.

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Cite this Article

Köster, D. V., Bhat, A., Talluri, S., Mayor, S. Reconstitution of Membrane-Tethered Minimal Actin Cortices on Supported Lipid Bilayers. J. Vis. Exp. (185), e63968, doi:10.3791/63968 (2022).

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