/
/
Using Microfluidics and Fluorescence Microscopy to Study the Assembly Dynamics of Single Actin Filaments and Bundles
A subscription to JoVE is required to view this content. Sign in or start your free trial.
2,321 Views
•
08:02 min
•
May 05, 2022
DOI:
Summary
Automatically generated
We present protocols for simple actin filament microfluidic assays, in combination with fluorescence microscopy, that allow one to accurately monitor individual actin filaments in real-time while sequentially exposing them to different protein solutions.
Read Article
Cite this Article
Wioland, H., Ghasemi, F., Chikireddy, J., Romet-Lemonne, G., Jégou, A. Using Microfluidics and Fluorescence Microscopy to Study the Assembly Dynamics of Single Actin Filaments and Bundles. J. Vis. Exp. (183), e63891, doi:10.3791/63891 (2022).
Copy