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生物化学
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Using Microfluidics and Fluorescence Microscopy to Study the Assembly Dynamics of Single Actin Filaments and Bundles
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生物化学
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JoVE 杂志 生物化学
Using Microfluidics and Fluorescence Microscopy to Study the Assembly Dynamics of Single Actin Filaments and Bundles

Using Microfluidics and Fluorescence Microscopy to Study the Assembly Dynamics of Single Actin Filaments and Bundles

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08:02 min

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May 05, 2022

DOI:

10.3791/63891-v

DOI:
10.3791/63891-v

•

08:02 min
May 05, 2022

•

1 Views
, , , ,
1Université Paris Cité, CNRS,Institut Jacques Monod

Summary

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We present protocols for simple actin filament microfluidic assays, in combination with fluorescence microscopy, that allow one to accurately monitor individual actin filaments in real-time while sequentially exposing them to different protein solutions.

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Cite this Article

Wioland, H., Ghasemi, F., Chikireddy, J., Romet-Lemonne, G., Jégou, A. Using Microfluidics and Fluorescence Microscopy to Study the Assembly Dynamics of Single Actin Filaments and Bundles. J. Vis. Exp. (183), e63891, doi:10.3791/63891 (2022).

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