Journal
/
Developmental Biology
/
Gerando esferas 3D e culturas de interface ar-líquido 2D de células-tronco pluripotentes induzidas pelo homem
JoVE Journal
Developmental Biology
This content is Free Access.
JoVE Journal Developmental Biology
Generating 3D Spheres and 2D Air-Liquid Interface Cultures of Human Induced Pluripotent Stem Cell-Derived Type 2 Alveolar Epithelial Cells

Gerando esferas 3D e culturas de interface ar-líquido 2D de células-tronco pluripotentes induzidas pelo homem

português

Automatically Generated

4,114 Views

•

07:02 min

•

April 15, 2022

DOI:

10.3791/63875-v

DOI:
10.3791/63875-v

•

07:02 min
April 15, 2022

•

14 Views
, , , , , , , , , , ,
1Center for Regenerative Medicine,Boston University and Boston Medical Center, 2The Pulmonary Center and Department of Medicine,Boston University School of Medicine, 3QIMR Berghofer Medical Research Institute

Summary

Automatically generated

O presente protocolo descreve células pluripotentes pluripotentes induzidas por células-tronco derivadas do tipo 2 (iAT2s). Essas células podem ser cultivadas como esferas de auto-renovação na cultura 3D ou adaptadas à cultura de interface ar-líquido (ALI).

Explore More Videos

3D Spheres2D Air-liquid InterfaceHuman Induced Pluripotent Stem Cell-derived Type 2 Alveolar Epithelial CellsLung BiologyAlveolospheresDispaseTrypsinAir-liquid Interface Culture3D MatrixCell CultureIAT2 Cells

Read Article

Cite this Article

Werder, R. B., Huang, J., Abo, K. M., Hix, O. T., Minakin, K., Alysandratos, K., Merritt, C., Berthiaume, K., Alber, A. B., Burgess, C. L., Kotton, D. N., Wilson, A. A. Generating 3D Spheres and 2D Air-Liquid Interface Cultures of Human Induced Pluripotent Stem Cell-Derived Type 2 Alveolar Epithelial Cells. J. Vis. Exp. (182), e63875, doi:10.3791/63875 (2022).

Download .ris file

Copy

Share Video

.

Copy