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发育生物学
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Generating 3D Spheres and 2D Air-Liquid Interface Cultures of Human Induced Pluripotent Stem Cell-Derived Type 2 Alveolar Epithelial Cells
JoVE 杂志
发育生物学
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JoVE 杂志 发育生物学
Generating 3D Spheres and 2D Air-Liquid Interface Cultures of Human Induced Pluripotent Stem Cell-Derived Type 2 Alveolar Epithelial Cells

Generating 3D Spheres and 2D Air-Liquid Interface Cultures of Human Induced Pluripotent Stem Cell-Derived Type 2 Alveolar Epithelial Cells

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07:02 min

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April 15, 2022

DOI:

10.3791/63875-v

DOI:
10.3791/63875-v

•

07:02 min
April 15, 2022

•

14 Views
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1Center for Regenerative Medicine,Boston University and Boston Medical Center, 2The Pulmonary Center and Department of Medicine,Boston University School of Medicine, 3QIMR Berghofer Medical Research Institute

Summary

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The present protocol describes human induced pluripotent stem cell-derived type 2 alveolar epithelial-like cells (iAT2s). These cells can be cultured as self-renewing spheres in 3D culture or adapted to air-liquid interface (ALI) culture.

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Cite this Article

Werder, R. B., Huang, J., Abo, K. M., Hix, O. T., Minakin, K., Alysandratos, K., Merritt, C., Berthiaume, K., Alber, A. B., Burgess, C. L., Kotton, D. N., Wilson, A. A. Generating 3D Spheres and 2D Air-Liquid Interface Cultures of Human Induced Pluripotent Stem Cell-Derived Type 2 Alveolar Epithelial Cells. J. Vis. Exp. (182), e63875, doi:10.3791/63875 (2022).

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