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Avance de imágenes de alta resolución de ensamblajes de virus en líquido y hielo
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08:31 min
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July 20, 2022
DOI:
1Department of Biomedical Engineering,Pennsylvania State University, 2Department of Materials Science and Engineering,McMaster University, 3Bioinformatics and Genomics Graduate Program, Huck Institutes of the Life Sciences,Pennsylvania State University, 4Molecular, Cellular, and Integrative Biosciences Graduate Program, Huck Institutes of the Life Sciences,Pennsylvania State University, 5Materials Research Institute,Pennsylvania State University, 6Huck Institutes of the Life Sciences,Pennsylvania State University, 7Applications team,Direct Electron, 8Application Scientist,Protochips, Inc., 9Department of Biology,Wake Forest University
Summary
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Aquí se describen los protocolos para preparar conjuntos de virus adecuados para el análisis de EM líquido y crio-EM a nanoescala utilizando microscopía electrónica de transmisión.
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Cite this Article
DiCecco, L., Berry, S., Jonaid, G. M., Solares, M. J., Kaylor, L., Gray, J. L., Bator, C., Dearnaley, W. J., Spilman, M., Dressel-Dukes, M. J., Grandfield, K., McDonald Esstman, S. M., Kelly, D. F. Advancing High-Resolution Imaging of Virus Assemblies in Liquid and Ice. J. Vis. Exp. (185), e63856, doi:10.3791/63856 (2022).
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