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Advancing High-Resolution Imaging of Virus Assemblies in Liquid and Ice
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JoVE Revista Bioquímica
Advancing High-Resolution Imaging of Virus Assemblies in Liquid and Ice

Advancing High-Resolution Imaging of Virus Assemblies in Liquid and Ice

DOI:
10.3791/63856-v

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08:31 min

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July 20, 2022

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, , , , , , , , , , , ,
1Department of Biomedical Engineering,Pennsylvania State University, 2Department of Materials Science and Engineering,McMaster University, 3Bioinformatics and Genomics Graduate Program, Huck Institutes of the Life Sciences,Pennsylvania State University, 4Molecular, Cellular, and Integrative Biosciences Graduate Program, Huck Institutes of the Life Sciences,Pennsylvania State University, 5Materials Research Institute,Pennsylvania State University, 6Huck Institutes of the Life Sciences,Pennsylvania State University, 7Applications team,Direct Electron, 8Application Scientist,Protochips, Inc., 9Department of Biology,Wake Forest University

Capítulos

  • 00:05Introduction
  • 01:43Loading the Specimen Holder for Liquid-EM
  • 02:47Production of Microchip Sandwich Assemblies
  • 03:51Imaging Specimens Using a Transmission Electron Microscope
  • 05:15Analysis of SARS-CoV-2 Sub-Viral Assemblies in Liquid
  • 06:09Results: Representative structures of AAV, SARS-CoV-2 Sub-Viral Assemblies and Rotavirus DLPs Prepared in Liquid and Vitreous Ice Using the Microchip Sandwich Technique
  • 07:24Conclusion

Summary

Traducción Automática

Traducción Automática

Here protocols are described to prepare virus assemblies suitable for liquid-EM and cryo-EM analysis at the nanoscale using transmission electron microscopy.

Tags

Liquid Electron MicroscopySARS-CoV-2ProteinsVitreous IceLiquid EnvironmentsVaccine CandidatesAntibody-based TherapiesMolecular DynamicsCryo-EMLiquid EM WorkflowsMicrochip Sandwich MethodSilicon Nitride MicrochipsGlow DischargeTurbo Pumped Dry Pumping Station

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