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Single-Molecule Analyse van Sf9 Purified Superprocessive Kinesin-3 Family Motoren
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Biochemistry
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JoVE Journal Biochemistry
Single-Molecule Analysis of Sf9 Purified Superprocessive Kinesin-3 Family Motors
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Single-Molecule Analyse van Sf9 Purified Superprocessive Kinesin-3 Family Motoren

Nederlands

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1,720 Views

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08:16 min

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July 27, 2022

DOI:

10.3791/63837-v

DOI:
10.3791/63837-v

•

08:16 min
July 27, 2022

•

2 Views
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1Discipline of Biological Engineering,Indian Institute of Technology Gandhinagar, 2Department of Biotechnology and Bioinformatics,Sambalpur University

Summary

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Deze studie beschrijft de zuivering van KIF1A (1-393LZ), een lid van de kinesine-3-familie, met behulp van het Sf9-baculovirusexpressiesysteem. In vitro single-molecule en multi-motor zweefanalyse van deze gezuiverde motoren vertoonde robuuste motiliteitseigenschappen vergelijkbaar met motoren van zoogdiercellysaat. Sf9-baculovirussysteem is dus vatbaar voor expressie en zuivering van motoreiwit van belang.

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Cite this Article

Soppina, P., Shewale, D. J., Naik, P. K., Soppina, V. Single-Molecule Analysis of Sf9 Purified Superprocessive Kinesin-3 Family Motors. J. Vis. Exp. (185), e63837, doi:10.3791/63837 (2022).

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