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Single-Molecule Analysis of Sf9 Purified Superprocessive Kinesin-3 Family Motors
JoVE Journal
Bioquímica
Author Produced
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JoVE Journal Bioquímica
Single-Molecule Analysis of Sf9 Purified Superprocessive Kinesin-3 Family Motors

Single-Molecule Analysis of Sf9 Purified Superprocessive Kinesin-3 Family Motors

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08:16 min

July 27, 2022

DOI:

08:16 min
July 27, 2022

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Summary

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This study details purification of KIF1A(1-393LZ), a member of kinesin-3 family, using Sf9-baculovirus expression system. In vitro single-molecule and multi-motor gliding analysis of these purified motors exhibited robust motility properties comparable to motors from mammalian cell lysate. Thus, Sf9-baculovirus system is amenable to express and purify motor protein of interest.

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