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Terapötik Hedef Olarak Polipeptitin Sodyum Taurokolat Kotransportasyonu Yoluyla Hepatit B Virüsü Girişini İnceleyen Yetkin Bir Hepatosit Modeli
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Biochemistry
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JoVE Journal Biochemistry
A Competent Hepatocyte Model Examining Hepatitis B Virus Entry through Sodium Taurocholate Cotransporting Polypeptide as a Therapeutic Target
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Terapötik Hedef Olarak Polipeptitin Sodyum Taurokolat Kotransportasyonu Yoluyla Hepatit B Virüsü Girişini İnceleyen Yetkin Bir Hepatosit Modeli

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1,976 Views

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11:34 min

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May 10, 2022

DOI:

10.3791/63761-v

DOI:
10.3791/63761-v

•

11:34 min
May 10, 2022

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8 Views
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1Department of Biochemistry, Faculty of Pharmacy,Mahidol University, 2Program in Translational Medicine, Faculty of Medicine Ramathibodi Hospital,Mahidol University, 3Excellent Center for Drug Discovery, Faculty of Science,Mahidol University, 4Department of Biotechnology, Faculty of Science,Mahidol University, 5Department of Pharmacology, Faculty of Medicine Siriraj Hospital,Mahidol University

Summary

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Konakçı sodyum taurokolat birlikte taşınan polipeptit ile bağlanma afinitesini (KD) ölçmek için izotermal titrasyon kalorimetrisini kullanarak, viral giriş öncesi ve sonrası yaşam döngüsü aşamalarını hedefleyen anti-hepatit B virüsü (HBV) bileşiklerini taramak için bir protokol sunuyoruz. Antiviral etkinlik, viral yaşam döngüsü belirteçlerinin (cccDNA oluşumu, transkripsiyon ve viral montaj) baskılanması yoluyla belirlendi.

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Sa-ngiamsuntorn, K., Thongsri, P., Pewkliang, Y., Borwornpinyo, S., Wongkajornsilp, A. A Competent Hepatocyte Model Examining Hepatitis B Virus Entry through Sodium Taurocholate Cotransporting Polypeptide as a Therapeutic Target. J. Vis. Exp. (183), e63761, doi:10.3791/63761 (2022).

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