/
/
A Competent Hepatocyte Model Examining Hepatitis B Virus Entry through Sodium Taurocholate Cotransporting Polypeptide as a Therapeutic Target
Se requiere una suscripción a JoVE para ver este contenido. Inicie sesión o comience su prueba gratuita.
A Competent Hepatocyte Model Examining Hepatitis B Virus Entry through Sodium Taurocholate Cotransporting Polypeptide as a Therapeutic Target
1,976 Views
•
11:34 min
•
May 10, 2022
DOI:
1Department of Biochemistry, Faculty of Pharmacy,Mahidol University, 2Program in Translational Medicine, Faculty of Medicine Ramathibodi Hospital,Mahidol University, 3Excellent Center for Drug Discovery, Faculty of Science,Mahidol University, 4Department of Biotechnology, Faculty of Science,Mahidol University, 5Department of Pharmacology, Faculty of Medicine Siriraj Hospital,Mahidol University
Summary
Automatically generated
We present a protocol to screen anti-hepatitis B virus (HBV) compounds targeting pre- and post-viral entry lifecycle stages, using isothermal titration calorimetry to measure binding affinity (KD) with host sodium taurocholate cotransporting polypeptide. Antiviral efficacy was determined through the suppression of viral lifecycle markers (cccDNA formation, transcription, and viral assembly).
Read Article
Cite this Article
Sa-ngiamsuntorn, K., Thongsri, P., Pewkliang, Y., Borwornpinyo, S., Wongkajornsilp, A. A Competent Hepatocyte Model Examining Hepatitis B Virus Entry through Sodium Taurocholate Cotransporting Polypeptide as a Therapeutic Target. J. Vis. Exp. (183), e63761, doi:10.3791/63761 (2022).
Copy