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组织清除后使用3D重建方法检测和定量小鼠门牙中的标记保留细胞

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Developmental Biology

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JoVE Journal Developmental Biology

Detection and Quantitation of Label-Retaining Cells in Mouse Incisors using a 3D Reconstruction Approach after Tissue Clearing

Please note that all translations are automatically generated. Click here for the English version.

组织清除后使用3D重建方法检测和定量小鼠门牙中的标记保留细胞

DOI:

10.3791/63721-v

•

10:32 min

•

June 10, 2022

•

Changchun Dong*¹, Bikash Lamichhane*¹, Yongxu Zhang, Xiaofang Wang

¹Department of Biomedical Sciences,Texas A&M School of Dentistry

Chapters

00:05Introduction
00:42Tissue Clearing of Mandible Using PEGASOS Technique
03:03Confocal Imaging of Tissue-Cleared Mandible
04:37Image Processing, 3D Image Reconstruction, and Quantification of Label-Retaining Cells
08:25Results: Detection and Quantitation of Label-Retaining Cells in Mouse Incisors
09:50Conclusion

Summary

Automatic Translation

小鼠门牙在其干细胞生态位中含有有价值的标记保留细胞。我们有一种新颖的方法来无偏地检测和量化标签保留细胞;我们的研究在PEGASOS组织清除下颌骨后使用了EdU标记和3D重建方法。

Tags

Keywords: 3D Reconstruction Label-retaining Cells Mouse Incisor Stem Cell Niche 5-ethynyl-2′-deoxyuridine (EdU) Staining Whole Mount Decalcification Decolorization De-lipidation Tert-butanol-polyethylene Glycol (tB-PEG)Benzyl Benzoate-polyethylene Glycol (BB-PEG) Clearing Confocal Imaging

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