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Detection and Quantitation of Label-Retaining Cells in Mouse Incisors using a 3D Reconstruction Approach after Tissue Clearing
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发育生物学
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Detection and Quantitation of Label-Retaining Cells in Mouse Incisors using a 3D Reconstruction Approach after Tissue Clearing

Detection and Quantitation of Label-Retaining Cells in Mouse Incisors using a 3D Reconstruction Approach after Tissue Clearing

1,677 Views

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10:32 min

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June 10, 2022

DOI:

10.3791/63721-v

DOI:
10.3791/63721-v

•

10:32 min
June 10, 2022

•

8 Views
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1Department of Biomedical Sciences,Texas A&M School of Dentistry

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3D ReconstructionLabel-retaining CellsMouse IncisorStem Cell Niche5-ethynyl-2′-deoxyuridine (EdU) StainingWhole MountDecalcificationDecolorizationDe-lipidationTert-butanol-polyethylene Glycol (tB-PEG)Benzyl Benzoate-polyethylene Glycol (BB-PEG) ClearingConfocal Imaging

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Dong, C., Lamichhane, B., Zhang, Y., Wang, X. Detection and Quantitation of Label-Retaining Cells in Mouse Incisors using a 3D Reconstruction Approach after Tissue Clearing. J. Vis. Exp. (184), e63721, doi:10.3791/63721 (2022).

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