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Size Exclusion Chromatography for Separating Extracellular Vesicles from Conditioned Cell Culture Media
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JoVE Journal Neuroscience
Size Exclusion Chromatography for Separating Extracellular Vesicles from Conditioned Cell Culture Media

Size Exclusion Chromatography for Separating Extracellular Vesicles from Conditioned Cell Culture Media

English

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3,151 Views

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10:46 min

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May 13, 2022

DOI:

10.3791/63614-v

DOI:
10.3791/63614-v

•

10:46 min
May 13, 2022

•

9 Views
, ,
1Department of Biology, School of Science,Penn State Erie, The Behrend College, 2Magee Womens Research Institute—Allied Member

Summary

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The protocol here demonstrates that extracellular vesicles can be adequately separated from conditioned cell culture media using size exclusion chromatography.

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Size Exclusion ChromatographyExtracellular VesiclesConditioned Cell Culture MediaSeparationCharacterizationIntracellular CommunicationUltracentrifugationFiltrationFraction Collection

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Jones, M. T., Manioci, S. W., Russell, A. E. Size Exclusion Chromatography for Separating Extracellular Vesicles from Conditioned Cell Culture Media. J. Vis. Exp. (183), e63614, doi:10.3791/63614 (2022).

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