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Size Exclusion Chromatography for Separating Extracellular Vesicles from Conditioned Cell Culture Media
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JoVE Journal 神経科学
Size Exclusion Chromatography for Separating Extracellular Vesicles from Conditioned Cell Culture Media

Size Exclusion Chromatography for Separating Extracellular Vesicles from Conditioned Cell Culture Media

DOI:
10.3791/63614-v

•

10:46 min

•

May 13, 2022

•

, ,
1Department of Biology, School of Science,Penn State Erie, The Behrend College, 2Magee Womens Research Institute—Allied Member

章

  • 00:05Introduction
  • 00:31Collection and Concentration of Conditioned Cell Culture Media
  • 02:51Extracellular Vesicle (EV) Separation Using Size Exclusion Chromatography
  • 06:02Ultrafiltration of EV and Protein Fractions
  • 07:52Results: Separating EVs from the Conditioned Cell Culture Media Using Size Exclusion Chromatography
  • 10:06Conclusion

概要

自動翻訳

自動翻訳

The protocol here demonstrates that extracellular vesicles can be adequately separated from conditioned cell culture media using size exclusion chromatography.

タグ

Size Exclusion ChromatographyExtracellular VesiclesConditioned Cell Culture MediaSeparationCharacterizationIntracellular CommunicationUltracentrifugationFiltrationFraction Collection

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