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September 16, 2022
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We present a protocol for the isolation and freeze-fracturing of extracellular vesicles (EVs) originating from cancerous urothelial cells. The freeze-fracture technique revealed the EVs' diameter and shape and-as a unique feature-the internal organization of the EV membranes. These are of immense importance in understanding how EVs interact with the recipient membranes.
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Resnik, N., Romih, R., Kreft, M. E., Hudoklin, S. Freeze-Fracture Electron Microscopy for Extracellular Vesicle Analysis. J. Vis. Exp. (187), e63550, doi:10.3791/63550 (2022).
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