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Freeze-Fracture Electron Microscopy for Extracellular Vesicle Analysis
 

Freeze-Fracture Electron Microscopy for Extracellular Vesicle Analysis

Article DOI: 10.3791/63550-v • 11:30 min • September 16th, 2022
September 16th, 2022 •
1, 1, 1, 1
1Faculty of Medicine, Institute of Cell Biology, University of Ljubljana

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We present a protocol for the isolation and freeze-fracturing of extracellular vesicles (EVs) originating from cancerous urothelial cells. The freeze-fracture technique revealed the EVs' diameter and shape and-as a unique feature-the internal organization of the EV membranes. These are of immense importance in understanding how EVs interact with the recipient membranes.

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Freeze-fracture Electron Microscopy Extracellular Vesicles Bladder Cancer Cells Intercellular Communication Exosomes Microvesicles Apoptotic Bodies Molecular Composition Limiting Membrane Recipient Cell Membrane Isolation Of Extracellular Vesicles Freeze-fracture Technique Two-dimensional Organization Of Biological Membranes Rapid Freezing Replica Making Transmission Electron Microscope Microvesicle Characterization
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