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Standard Membrane Feeding Assay for the Detection of Plasmodium falciparum Infection in Anopheles Mosquito Vectors
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Immunology and Infection
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JoVE Journal Immunology and Infection
Standard Membrane Feeding Assay for the Detection of Plasmodium falciparum Infection in Anopheles Mosquito Vectors

Standard Membrane Feeding Assay for the Detection of Plasmodium falciparum Infection in Anopheles Mosquito Vectors

DOI:
10.3791/63546-v

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05:28 min

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May 12, 2022

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, ,
1Wits Research Institute for Malaria, School of Pathology, Faculty of Health Sciences,University of the Witwatersrand, 2Centre for Emerging Zoonotic and Parasitic Diseases, National Institute for Communicable Diseases,National Health Laboratory Service

Chapters

  • 00:04Introduction
  • 00:49Artificial Infection of Mosquitoes through the SMFA
  • 02:28Preparation and Dissection of Infected Mosquitoes
  • 03:31Results: Identification and Assessment of Potential Antimalarial Compounds
  • 04:28Conclusion

Summary

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The standard membrane feeding assay (SMFA) is regarded as the gold standard for the assessment and identification of potential antimalarial compounds. This artificial feeding system is used to infect mosquitoes to further evaluate the effects of such compounds on the intensity and prevalence of the Plasmodium falciparum parasite.

Tags

Plasmodium FalciparumAnopheles MosquitoMembrane Feeding AssayMalaria TransmissionGametocyte-infected BloodMosquito InfectionOocyst DetectionMidgut DissectionLaboratory ScreeningTransmission-blocking Compounds

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