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Standard Membrane Feeding Assay for the Detection of Plasmodium falciparum Infection in Anopheles Mosquito Vectors
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Immunologie et infection
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Journal JoVE Immunologie et infection
Standard Membrane Feeding Assay for the Detection of Plasmodium falciparum Infection in Anopheles Mosquito Vectors

Standard Membrane Feeding Assay for the Detection of Plasmodium falciparum Infection in Anopheles Mosquito Vectors

2,408 Views

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05:28 min

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May 12, 2022

DOI:

10.3791/63546-v

DOI:
10.3791/63546-v

•

05:28 min
May 12, 2022

•

20 Views
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1Wits Research Institute for Malaria, School of Pathology, Faculty of Health Sciences,University of the Witwatersrand, 2Centre for Emerging Zoonotic and Parasitic Diseases, National Institute for Communicable Diseases,National Health Laboratory Service

Summary

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The standard membrane feeding assay (SMFA) is regarded as the gold standard for the assessment and identification of potential antimalarial compounds. This artificial feeding system is used to infect mosquitoes to further evaluate the effects of such compounds on the intensity and prevalence of the Plasmodium falciparum parasite.

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Plasmodium FalciparumAnopheles MosquitoMembrane Feeding AssayMalaria TransmissionGametocyte-infected BloodMosquito InfectionOocyst DetectionMidgut DissectionLaboratory ScreeningTransmission-blocking Compounds

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Erlank, E., Venter, N., Koekemoer, L. L. Standard Membrane Feeding Assay for the Detection of Plasmodium falciparum Infection in Anopheles Mosquito Vectors. J. Vis. Exp. (183), e63546, doi:10.3791/63546 (2022).

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