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基于荧光的膜电位测定，用于两个上皮细胞系中两个内源性离子通道的高通量功能研究
JoVE Journal
Biology
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JoVE Journal Biology
A Fluorescence-Based Assay of Membrane Potential for High-Throughput Functional Study of Two Endogenous Ion Channels in Two Epithelial Cell Lines
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基于荧光的膜电位测定，用于两个上皮细胞系中两个内源性离子通道的高通量功能研究

English

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1,600 Views

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06:59 min

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June 22, 2022

DOI:

10.3791/63528-v

DOI:
10.3791/63528-v

•

06:59 min
June 22, 2022

•

10 Views
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1Molecular Medicine,Hospital for Sick Children, 2Cell Biology,Hospital for Sick Children, 3Department of Physiology,University of Toronto, 4Department of Paediatrics,University of Toronto, 5Department of Biochemistry,University of Toronto

Summary

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该协议描述了通过测量膜电位变化来研究电源膜蛋白的方法。该测定为上皮细胞系中内源性表达的多个离子通道的功能读数提供了一个平台。

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Cite this Article

Xia, S., Di Paola, M., Jones, N. L., Bear, C. E. A Fluorescence-Based Assay of Membrane Potential for High-Throughput Functional Study of Two Endogenous Ion Channels in Two Epithelial Cell Lines. J. Vis. Exp. (184), e63528, doi:10.3791/63528 (2022).

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