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A Fluorescence-Based Assay of Membrane Potential for High-Throughput Functional Study of Two Endogenous Ion Channels in Two Epithelial Cell Lines
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Biology
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A Fluorescence-Based Assay of Membrane Potential for High-Throughput Functional Study of Two Endogenous Ion Channels in Two Epithelial Cell Lines

A Fluorescence-Based Assay of Membrane Potential for High-Throughput Functional Study of Two Endogenous Ion Channels in Two Epithelial Cell Lines

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1,661 Views

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06:59 min

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June 22, 2022

DOI:

10.3791/63528-v

DOI:
10.3791/63528-v

•

06:59 min
June 22, 2022

•

10 Views
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1Molecular Medicine,Hospital for Sick Children, 2Cell Biology,Hospital for Sick Children, 3Department of Physiology,University of Toronto, 4Department of Paediatrics,University of Toronto, 5Department of Biochemistry,University of Toronto

Summary

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This protocol describes a method for the study of electrogenic membrane proteins by measuring changes in membrane potential. This assay provides a platform for the functional readout of multiple ion channels endogenously expressed in epithelial cell lines.

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Xia, S., Di Paola, M., Jones, N. L., Bear, C. E. A Fluorescence-Based Assay of Membrane Potential for High-Throughput Functional Study of Two Endogenous Ion Channels in Two Epithelial Cell Lines. J. Vis. Exp. (184), e63528, doi:10.3791/63528 (2022).

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