A subscription to JoVE is required to view this content. Sign in or start your free trial.
2,007 Views
•
08:09 min
•
June 02, 2022
DOI:
Summary
Automatically generated
我们提出了一种电生理学表征双稳定光色素的方案：（i）利用光子吸收后光色素分子内的电荷位移及其在光感受器中的大量电荷，以及（ii）利用视紫红质和偏视紫红质光色素状态的吸收光谱差异。这些方案可用于筛选影响双稳定光色素系统的突变。
Explore More Videos
ElectrophysiologicalPhotopigmentDrosophilaPhotoreceptorsPhototransductionGenetic ScreenIn VivoElectrophysiologyRecording ElectrodeGround ElectrodeClampexAmplifierMaster-8Xenon LampShutter ControllerGlass CapillaryRinger SolutionPlatinum-iridium FilamentWaxAnesthetized FliesFly HolderFaraday CageMagnet BlockLight Guide
Read Article
Cite this Article
Gutorov, R., Katz, B., Minke, B. Electrophysiological Methods for Measuring Photopigment Levels in Drosophila Photoreceptors. J. Vis. Exp. (184), e63514, doi:10.3791/63514 (2022).
Copy