/
/
Electrophysiological Methods for Measuring Photopigment Levels in Drosophila Photoreceptors
É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
Electrophysiological Methods for Measuring Photopigment Levels in Drosophila Photoreceptors
2,027 Views
•
08:09 min
•
June 02, 2022
DOI:
Summary
Automatically generated
We present a protocol to electrophysiologically characterize bi-stable photopigments: (i) exploiting the charge displacements within the photopigment molecules following photon-absorption and their huge amount in the photoreceptors, and (ii) exploiting the absorption-spectra differences of rhodopsin and metarhodopsin photopigment states. These protocols are useful to screen for mutations affecting bi-stable photopigment systems.
Explore More Videos
ElectrophysiologicalPhotopigmentDrosophilaPhotoreceptorsPhototransductionGenetic ScreenIn VivoElectrophysiologyRecording ElectrodeGround ElectrodeClampexAmplifierMaster-8Xenon LampShutter ControllerGlass CapillaryRinger SolutionPlatinum-iridium FilamentWaxAnesthetized FliesFly HolderFaraday CageMagnet BlockLight Guide
Read Article
Cite this Article
Gutorov, R., Katz, B., Minke, B. Electrophysiological Methods for Measuring Photopigment Levels in Drosophila Photoreceptors. J. Vis. Exp. (184), e63514, doi:10.3791/63514 (2022).
Copy