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Electrophysiological Methods for Measuring Photopigment Levels in Drosophila Photoreceptors
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Neurosciences
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Journal JoVE Neurosciences
Electrophysiological Methods for Measuring Photopigment Levels in Drosophila Photoreceptors

Electrophysiological Methods for Measuring Photopigment Levels in Drosophila Photoreceptors

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08:09 min

June 02, 2022

DOI:

08:09 min
June 02, 2022

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Summary

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We present a protocol to electrophysiologically characterize bi-stable photopigments: (i) exploiting the charge displacements within the photopigment molecules following photon-absorption and their huge amount in the photoreceptors, and (ii) exploiting the absorption-spectra differences of rhodopsin and metarhodopsin photopigment states. These protocols are useful to screen for mutations affecting bi-stable photopigment systems. 

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