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Detektion av postreplikativa luckor ackumulering och reparation i mänskliga celler med hjälp av DNA-fiberanalysen
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Genetics
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JoVE Journal Genetics
Detection of Post-Replicative Gaps Accumulation and Repair in Human Cells Using the DNA Fiber Assay
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Detektion av postreplikativa luckor ackumulering och reparation i mänskliga celler med hjälp av DNA-fiberanalysen

4,590 Views

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10:32 min

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February 03, 2022

DOI:

10.3791/63448-v

DOI:
10.3791/63448-v

•

10:32 min
February 03, 2022

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11 Views
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1Department of Microbiology, Institute of Biomedical Sciences,University of São Paulo, 2Division of Oncology, Department of Internal Medicine,Washington University in St. Louis

Tags

DNA Fiber AssayPost-replicative GapsS1 NucleaseDNA RepairReplication StressGenome IntegrityUV-C IrradiationCldU IncorporationCSK PermeabilizationDNA Lesion Detection

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Martins, D. J., Tirman, S., Quinet, A., Menck, C. F. M. Detection of Post-Replicative Gaps Accumulation and Repair in Human Cells Using the DNA Fiber Assay. J. Vis. Exp. (180), e63448, doi:10.3791/63448 (2022).

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