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Culturing and Screening the Plant Parasitic Nematode Ditylenchus dipsaci
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Biology
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JoVE Journal Biology
Culturing and Screening the Plant Parasitic Nematode Ditylenchus dipsaci

Culturing and Screening the Plant Parasitic Nematode Ditylenchus dipsaci

DOI:
10.3791/63438-v

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08:04 min

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January 31, 2022

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, ,
1Department of Molecular Genetics,University of Toronto, 2Department of Biochemistry,University of Toronto, 3The Donnelly Centre for Cellular and Biomolecular Research,University of Toronto, 4Department of Pharmacology and Toxicology,University of Toronto

Chapters

  • 00:05Introduction
  • 00:38Culturing of D. dipsaci
  • 03:22Extraction and Collection of D. dipsaci
  • 04:14In Vitro Small Molecule Screen
  • 05:36Data Collection and Analysis
  • 06:13Results: In Vitro Small Molecule Screen Assay for Candidate Nematicides
  • 07:40Conclusion

Summary

Automatic Translation

Automatic Translation

The present protocol describes a reliable and straightforward method for culturing, collecting, and screening Ditylenchus dipsaci.

Tags

Plant Parasitic NematodeDitylenchus DipsaciNematicidesCulturingMedium-throughputChemical ScreensNutrient AgarGamborg B5 MediaSeed SterilizationPea SeedsSucrose Solution

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