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Culturing and Screening the Plant Parasitic Nematode Ditylenchus dipsaci
JoVE 杂志
生物学
This content is Free Access.
JoVE 杂志 生物学
Culturing and Screening the Plant Parasitic Nematode Ditylenchus dipsaci

Culturing and Screening the Plant Parasitic Nematode Ditylenchus dipsaci

DOI:
10.3791/63438-v

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08:04 min

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January 31, 2022

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, ,
1Department of Molecular Genetics,University of Toronto, 2Department of Biochemistry,University of Toronto, 3The Donnelly Centre for Cellular and Biomolecular Research,University of Toronto, 4Department of Pharmacology and Toxicology,University of Toronto

Chapters

  • 00:05Introduction
  • 00:38Culturing of D. dipsaci
  • 03:22Extraction and Collection of D. dipsaci
  • 04:14In Vitro Small Molecule Screen
  • 05:36Data Collection and Analysis
  • 06:13Results: In Vitro Small Molecule Screen Assay for Candidate Nematicides
  • 07:40Conclusion

Summary

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The present protocol describes a reliable and straightforward method for culturing, collecting, and screening Ditylenchus dipsaci.

Tags

Plant Parasitic NematodeDitylenchus DipsaciNematicidesCulturingMedium-throughputChemical ScreensNutrient AgarGamborg B5 MediaSeed SterilizationPea SeedsSucrose Solution

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