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Simultaneous Visualization of the Dynamics of Crosslinked and Single Microtubules In Vitro by TIRF Microscopy
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Biochemistry
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JoVE Journal Biochemistry
Simultaneous Visualization of the Dynamics of Crosslinked and Single Microtubules In Vitro by TIRF Microscopy

Simultaneous Visualization of the Dynamics of Crosslinked and Single Microtubules In Vitro by TIRF Microscopy

English

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2,359 Views

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07:20 min

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February 18, 2022

DOI:

10.3791/63377-v

DOI:
10.3791/63377-v

•

07:20 min
February 18, 2022

•

2 Views
, ,
1Molecular Biology,Massachusetts General Hospital, 2Department of Genetics,Harvard Medical School

Summary

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Here, a TIRF microscopy-based in vitro reconstitution assay is presented to simultaneously quantify and compare the dynamics of two microtubule populations. A method is described to simultaneously view the collective activity of multiple microtubule-associated proteins on crosslinked microtubule bundles and single microtubules.

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Mani, N., Marchan, M. F., Subramanian, R. Simultaneous Visualization of the Dynamics of Crosslinked and Single Microtubules In Vitro by TIRF Microscopy. J. Vis. Exp. (180), e63377, doi:10.3791/63377 (2022).

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