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Simultaneous Visualization of the Dynamics of Crosslinked and Single Microtubules In Vitro by TIRF Microscopy
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Simultaneous Visualization of the Dynamics of Crosslinked and Single Microtubules In Vitro by TIRF Microscopy
Capítulos
- 00:04Introduction
- 01:05Microtubule Visualization via TIRF Microscopy
- 01:58Microscope Settings
- 03:17Generation of Surface-Immobilized Microtubule Bundles
- 04:57Image Microtubule Dynamics
- 05:53Results: TIRF Microscopy of Crosslinked and Single Microtubules
- 06:35Conclusion
Here, a TIRF microscopy-based in vitro reconstitution assay is presented to simultaneously quantify and compare the dynamics of two microtubule populations. A method is described to simultaneously view the collective activity of multiple microtubule-associated proteins on crosslinked microtubule bundles and single microtubules.
