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Therapeutic Evaluation of Fecal Microbiota Transplantation in an Interleukin 10-Deficient Mouse Model
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Immunology and Infection
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JoVE Journal Immunology and Infection
Therapeutic Evaluation of Fecal Microbiota Transplantation in an Interleukin 10-Deficient Mouse Model

Therapeutic Evaluation of Fecal Microbiota Transplantation in an Interleukin 10-Deficient Mouse Model

DOI:
10.3791/63350-v

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05:41 min

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April 06, 2022

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, , ,
1Division of Gastroenterology, Department of Internal Medicine,University of Texas Medical Branch at Galveston, 2Department of Gastroenterology,Xiangya Hospital of Central South University

Chapters

  • 00:04Introduction
  • 00:51Collection of Fresh Fecal Pellets
  • 02:02Preparation of Fecal Suspension
  • 02:45Administration of Fecal Suspension by Oral Gavage
  • 04:24Results: BNP Up-Regulation and LVEF Down-Regulation Mitigation by FMT
  • 05:09Conclusion

Summary

Automatic Translation

Automatic Translation

The interaction of genetic susceptibility, mucosal immunity, and intestinal microecological environment is involved in the pathogenesis of inflammatory bowel disease (IBD). In this study, we applied fecal microbiota transplantation to IL-10 deficient mice and investigated its impact on colonic inflammation and heart function.

Tags

Fecal Microbiota TransplantationIL-10 Deficient Mouse ModelOral GavageSterile TechniqueFecal DonorFecal SuspensionHomogenizationFiltrationCryogenic StorageThawingGavage Needle

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