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Neural Stem Cell Reactivation in Cultured Drosophila Brain Explants
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Neuroscience
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JoVE Journal Neuroscience
Neural Stem Cell Reactivation in Cultured Drosophila Brain Explants

Neural Stem Cell Reactivation in Cultured Drosophila Brain Explants

English

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2,899 Views

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05:54 min

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May 18, 2022

DOI:

10.3791/63189-v

DOI:
10.3791/63189-v

•

05:54 min
May 18, 2022

•

1 Views
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1Biology department,University of Virginia

Summary

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A method to reactivate quiescent neural stem cells in cultured Drosophila brain explants has been established. Using this method, the role of systemic signals can be uncoupled from tissue-intrinsic signals in the regulation of neural stem cell quiescence, entry and exit.

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Neural Stem CellsDrosophilaBrain ExplantsStem Cell ReactivationExtrinsic CuesCell CycleDissectionSchneider’s Culture Medium5-ethynyl-2′-deoxyuridine (EdU)

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Cite this Article

Naomi Keliinui, C., Doyle, S. E., Siegrist, S. E. Neural Stem Cell Reactivation in Cultured Drosophila Brain Explants. J. Vis. Exp. (183), e63189, doi:10.3791/63189 (2022).

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