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Neural Stem Cell Reactivation in Cultured Drosophila Brain Explants
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Neurociência
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JoVE Journal Neurociência
Neural Stem Cell Reactivation in Cultured Drosophila Brain Explants

Neural Stem Cell Reactivation in Cultured Drosophila Brain Explants

DOI:
10.3791/63189-v

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05:54 min

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May 18, 2022

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, ,
1Biology department,University of Virginia

Capítulos

  • 00:04Introduction
  • 00:35Drosophila Larvae Collection
  • 01:23Culture Media and Tool Preparation
  • 02:03Dissections and Brain Cultures
  • 02:57Proliferation Assay, Brain Fixation, and Antibody Staining
  • 04:24Results: Confocal Imaging of Stained Brain Cells
  • 05:19Conclusion

Summary

Tadução automática

Tadução automática

A method to reactivate quiescent neural stem cells in cultured Drosophila brain explants has been established. Using this method, the role of systemic signals can be uncoupled from tissue-intrinsic signals in the regulation of neural stem cell quiescence, entry and exit.

Tags

Neural Stem CellsDrosophilaBrain ExplantsStem Cell ReactivationExtrinsic CuesCell CycleDissectionSchneider’s Culture Medium5-ethynyl-2′-deoxyuridine (EdU)

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