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Cromatografia ultra-alta-performance semi-direcionada acoplada à análise de espectrometria de massa de metabólitos fenólicos no plasma de idosos adultos
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April 22, 2022
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O objetivo deste protocolo é detectar metabólitos fenólicos no plasma usando um método de espectrometria de massa cromatografia-masscamo semi-direcionada.
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Semi-targetedUltra-high-performance ChromatographyMass SpectrometryPhenolic MetabolitesPlasmaElderly AdultsUPLC-MS/MSProtein PrecipitationMetabolite IdentificationBrosimum AlicastrumBioavailabilityBiotransformationUntargeted AnalysisSemi-targeted AnalysisMolecular FeaturesMass-to-charge RatioIsotopic DistributionFragmentation PatternPolyphenol Metabolites
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Muñoz-Bernal, Ó. A., Vazquez-Flores, A. A., Alvarez-Parrilla, E., Martínez-Ruiz, N. R., de la Rosa, L. A. Semi-Targeted Ultra-High-Performance Chromatography Coupled to Mass Spectrometry Analysis of Phenolic Metabolites in Plasma of Elderly Adults. J. Vis. Exp. (182), e63164, doi:10.3791/63164 (2022).
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