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高齢者の血漿中のフェノール代謝産物の質量分析に組み合わせた半標的超高速クロマトグラフィー
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Biochemistry
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JoVE Journal Biochemistry
Semi-Targeted Ultra-High-Performance Chromatography Coupled to Mass Spectrometry Analysis of Phenolic Metabolites in Plasma of Elderly Adults
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高齢者の血漿中のフェノール代謝産物の質量分析に組み合わせた半標的超高速クロマトグラフィー

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3,742 Views

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14:39 min

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April 22, 2022

DOI:

10.3791/63164-v

DOI:
10.3791/63164-v

•

14:39 min
April 22, 2022

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14 Views
, , , ,
1Department of Chemical Biological Sciences,Universidad Autónoma de Ciudad Juárez

Summary

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このプロトコルの目的は、半標的クロマトグラフィー質量分析法を使用して血漿中のフェノール代謝産物を検出することです。

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Semi-targetedUltra-high-performance ChromatographyMass SpectrometryPhenolic MetabolitesPlasmaElderly AdultsUPLC-MS/MSProtein PrecipitationMetabolite IdentificationBrosimum AlicastrumBioavailabilityBiotransformationUntargeted AnalysisSemi-targeted AnalysisMolecular FeaturesMass-to-charge RatioIsotopic DistributionFragmentation PatternPolyphenol Metabolites

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Cite this Article

Muñoz-Bernal, Ó. A., Vazquez-Flores, A. A., Alvarez-Parrilla, E., Martínez-Ruiz, N. R., de la Rosa, L. A. Semi-Targeted Ultra-High-Performance Chromatography Coupled to Mass Spectrometry Analysis of Phenolic Metabolites in Plasma of Elderly Adults. J. Vis. Exp. (182), e63164, doi:10.3791/63164 (2022).

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