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February 10, 2022
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The interaction of an ATP-dependent chromatin remodeler with a DNA ligand is described using CD spectroscopy. The induced conformational changes on a gene promoter analyzed by the peaks generated can be used to understand the mechanism of transcriptional regulation.
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Arya, V., Dutta, A., Muthuswami, R. CD Spectroscopy to Study DNA-Protein Interactions. J. Vis. Exp. (180), e63147, doi:10.3791/63147 (2022).
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