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CD Spectroscopy to Study DNA-Protein Interactions
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JoVE Revista Bioquímica
CD Spectroscopy to Study DNA-Protein Interactions

CD Spectroscopy to Study DNA-Protein Interactions

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06:48 min

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February 10, 2022

DOI:

10.3791/63147-v

DOI:
10.3791/63147-v

•

06:48 min
February 10, 2022

•

23 Views
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1Jawaharlal Nehru University New Delhi

Summary

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The interaction of an ATP-dependent chromatin remodeler with a DNA ligand is described using CD spectroscopy. The induced conformational changes on a gene promoter analyzed by the peaks generated can be used to understand the mechanism of transcriptional regulation.

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CD SpectroscopyDNA-protein InteractionsATP-dependent Chromatin RemodelingTranscription RegulationNADH Coupled Oxidation AssayDNA StructureOligonucleotidesQuartz CuvettesCD SpectraBaseline SpectraExperimental Reactions

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Cite this Article

Arya, V., Dutta, A., Muthuswami, R. CD Spectroscopy to Study DNA-Protein Interactions. J. Vis. Exp. (180), e63147, doi:10.3791/63147 (2022).

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