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Preparation of Bead-supported Lipid Bilayers to Study the Particulate Output of T Cell Immune Synapses
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Immunology and Infection
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JoVE Journal Immunology and Infection
Preparation of Bead-supported Lipid Bilayers to Study the Particulate Output of T Cell Immune Synapses

Preparation of Bead-supported Lipid Bilayers to Study the Particulate Output of T Cell Immune Synapses

English

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3,820 Views

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11:06 min

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April 01, 2022

DOI:

10.3791/63130-v

DOI:
10.3791/63130-v

•

11:06 min
April 01, 2022

•

33 Views
,
1Kennedy Institute of Rheumatology, Nuffield Department of Orthopaedics, Rheumatology and Musculoskeletal Sciences,The University of Oxford

Summary

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Here we present the protocol for the stepwise reconstitution of synthetic antigen-presenting cells using Bead-Supported Lipid Bilayers and their use to interrogate the synaptic output from activated T cells.

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Bead-supported Lipid BilayersT Cell Immune SynapsesFlow CytometryMicroscopyProteomicsRNA SequencingChimeric Antigen ReceptorsProtein CalibrationMulticolor Flow CytometrySilica BeadsLiposome Master MixNickel-containing PhospholipidsSterile TechniqueBSA Blocking SolutionHBS-HSA BufferSerial Dilutions

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Céspedes, P. F., Dustin, M. L. Preparation of Bead-supported Lipid Bilayers to Study the Particulate Output of T Cell Immune Synapses. J. Vis. Exp. (182), e63130, doi:10.3791/63130 (2022).

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