Rivista
/
Immunologia e infezioni
/
Preparation of Bead-supported Lipid Bilayers to Study the Particulate Output of T Cell Immune Synapses
JoVE Journal
Immunologia e infezioni
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Immunologia e infezioni
Preparation of Bead-supported Lipid Bilayers to Study the Particulate Output of T Cell Immune Synapses

Preparation of Bead-supported Lipid Bilayers to Study the Particulate Output of T Cell Immune Synapses

3,820 Views

•

11:06 min

•

April 01, 2022

DOI:

10.3791/63130-v

DOI:
10.3791/63130-v

•

11:06 min
April 01, 2022

•

33 Views
,
1Kennedy Institute of Rheumatology, Nuffield Department of Orthopaedics, Rheumatology and Musculoskeletal Sciences,The University of Oxford

Summary

Automatically generated

Here we present the protocol for the stepwise reconstitution of synthetic antigen-presenting cells using Bead-Supported Lipid Bilayers and their use to interrogate the synaptic output from activated T cells.

Explore More Videos

Bead-supported Lipid BilayersT Cell Immune SynapsesFlow CytometryMicroscopyProteomicsRNA SequencingChimeric Antigen ReceptorsProtein CalibrationMulticolor Flow CytometrySilica BeadsLiposome Master MixNickel-containing PhospholipidsSterile TechniqueBSA Blocking SolutionHBS-HSA BufferSerial Dilutions

Read Article

Cite this Article

Céspedes, P. F., Dustin, M. L. Preparation of Bead-supported Lipid Bilayers to Study the Particulate Output of T Cell Immune Synapses. J. Vis. Exp. (182), e63130, doi:10.3791/63130 (2022).

Download .ris file

Copy

Share Video

.

Copy