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Bioengineering
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使用水凝胶图案化技术控制细胞粘附,用于牵引力显微镜的应用
JoVE Journal
Bioengineering
Author Produced
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JoVE Journal
Bioengineering
Control of Cell Adhesion using Hydrogel Patterning Techniques for Applications in Traction Force Microscopy
Please note that all translations are automatically generated.
Click here for the English version.
使用水凝胶图案化技术控制细胞粘附,用于牵引力显微镜的应用
DOI:
10.3791/63121-v
•
12:26 min
•
January 29, 2022
•
Joel Christian
,
Johannes W. Blumberg
,
Dimitri Probst
,
Cristina Lo Giudice
,
Sandra Sindt
,
Christine Selhuber-Unkel
,
Ulrich S. Schwarz
,
Elisabetta Ada Cavalcanti-Adam
1
Department of Cellular Biophysics
,
Max Planck Institute for Medical Research
,
2
Institute for Theoretical Physics and BioQuant
,
Heidelberg University
,
3
Institute for Molecular Systems Engineering (IMSE)
,
Heidelberg University
Chapters
00:04
Introduction
01:22
Preparation of Methacrylated Coverslips
02:39
Micropatterning of Extracellular Matrix Proteins on Glass Coverslips
05:31
Fabrication of Patterned Polyacrylamide Hydrogels
09:15
Local Release of Cell Traction Forces by UV-A Laser Illumination
10:26
Image Processing, Particle Image Velocity and Calculation of Traction Forces
10:55
Results
11:56
Conclusion
Summary
Automatic Translation
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Automatic Translation
结合近紫外光刻和牵引力显微镜来测量微图案水凝胶上的细胞力。单细胞的靶向光诱导释放可以对同一样品进行大量测量。
Tags
Hydrogel Patterning
Traction Force Microscopy
Polyacrylamide Hydrogel
Micro-pattern
Extracellular Matrix
Fluorescent Beads
Cell Adhesion
Methacrylated Glass Cover Slip
Poly-L-Lysine
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