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Control of Cell Adhesion using Hydrogel Patterning Techniques for Applications in Traction Force Microscopy
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Biotechnik
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JoVE Journal Biotechnik
Control of Cell Adhesion using Hydrogel Patterning Techniques for Applications in Traction Force Microscopy

Control of Cell Adhesion using Hydrogel Patterning Techniques for Applications in Traction Force Microscopy

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12:26 min

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January 29, 2022

DOI:

10.3791/63121-v

DOI:
10.3791/63121-v

•

12:26 min
January 29, 2022

•

8 Views
, , , , , , ,
1Department of Cellular Biophysics,Max Planck Institute for Medical Research, 2Institute for Theoretical Physics and BioQuant,Heidelberg University, 3Institute for Molecular Systems Engineering (IMSE),Heidelberg University

Summary

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Near-UV lithography and traction force microscopy are combined to measure cellular forces on micropatterned hydrogels. The targeted light-induced release of single cells enables a high number of measurements on the same sample.

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Christian, J., Blumberg, J. W., Probst, D., Lo Giudice, C., Sindt, S., Selhuber-Unkel, C., Schwarz, U. S., Cavalcanti-Adam, E. A. Control of Cell Adhesion using Hydrogel Patterning Techniques for Applications in Traction Force Microscopy. J. Vis. Exp. (179), e63121, doi:10.3791/63121 (2022).

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